منابع مشابه
RNA-primed PCR.
We show that RNA can serve as a primer in PCR. Use of rTth DNA polymerase is essential because it has strong reverse transcriptase activity. RNA primers can be obtained by in vitro transcription and are less costly than DNA primers, which are chemically synthesized. RNA-primed PCR also opens the possibility that a specific amplification reaction can be achieved in the absence of knowledge of th...
متن کاملRNA: primed for packing?
How does RNA's seemingly subtle 2'-hydroxyl group contribute to its diverse biological functions? Using quantitative structure-activity relationship (QSAR) analysis, Piccirilli and colleagues find that the 2'-OH fosters van der Waals and hydrophobic interactions that play underappreciated roles in RNA structure and molecular recognition.
متن کاملWhole-Genome Amplification by Degenerate Oligonucleotide Primed PCR (DOP-PCR).
INTRODUCTIONPCR-based whole-genome amplification (WGA) has the goal of generating microgram quantities of genome-representative DNA from picogram or nanogram amounts of starting material. This amplification should introduce little, or ideally no, representational bias. Unlike other techniques for WGA, PCR-based methods are generally less affected by DNA quality and are more applicable to DNA ex...
متن کاملGene walking by unpredictably primed PCR.
Several methods have been described for gene walking by PCR on uncloned DNA (2 — 13). In general, they suffer from limitations: they either avoid high-complexity whole genomes, require delicate manipulation or give contaminated products. This report describes a reliable and simple PCR (1) adaptation for gene walks from any specific genomic locus: unpredictably primed PCR (UP-PCR). Besides its s...
متن کاملThe schistosome genome project: RNA arbitrarily primed PCR allows the accelerated generation of expressed sequence tags.
Centro de Pesquisas René Rachou FIOCRUZ, Av. Augusto de Lima 1715, 30190-002 Belo Horizonte, MG Brasil *Departamento de Bioquímica e Imunologia, ICB-UFMG, Caixa Postal 486, 30161970 Belo Horizonte, MG, Brasil **Department of Biology, University of York, Heslington, York, Y015DD, UK ***Laboratório de Genética de Câncer, Instituto Ludwig de Pesquisas Contra o Câncer, R. Prof. Antônio Prudente 109...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genome Research
سال: 1995
ISSN: 1088-9051
DOI: 10.1101/gr.5.4.400